In an attempt to discriminate between organic matter sources and reconstruct further the paleoenvironment of the southwestern coastal plain of Taiwan, organic carbon and nitrogen content (C/N) ratios and isotope composition (δ13C and δ15N) are measured for sedimentary core samples collected from San-liao-wan, Zai-kang and Yihju in the southwestern coastal plain of Taiwan. Heavy δ13C values (up to -14.3 ‰) and high C/N ratios (average 9.3) with good correlation indicate that this area underwent a period of relative aridity from early Marine Isotopic Stage (MIS) 2 to the early MIS 1. In MIS 1, the δ13C and C/N ratio both vary initially before later steadying, indicating that the environment became progressively more stable, with C3 plants becoming dominant again. The Zai-kang core appears to have come from a shallow lagoon system that potentially preserved the record of δ15N from autochthonous primary producers. Regional organic source variation is revealed through the high δ15N values and constant C/N ratios in MIS 1 of the Zai-kang core. This may suggest that mangroves and macrophytes were the main aquatic organic matter source in the Zai-kang area during MIS 1.